Electrochemical microsensor system for cancer research on photodynamic therapy in vitro

[摘要] An electrochemical microsensor system to investigate photodynamic therapy of cancer cells in vitro was developed and applied to monitor the cellular respiration during and after photodynamic therapy. The redox activity and therefore influence of the photodynamic drug on the sensor performance was investigated by electrochemical characterization. It was shown, that appropriate operation conditions avoid cross-sensitivity of the sensors to the drug itself. The presented system features a cell culture chamber equipped with microsensors and a laser source to photodynamically treat the cells while simultaneous monitoring of metabolic parameter in situ. Additionally, the optical setup allows to read back fluorescence signals from the photosensitizer itself or other marker molecules parallel to the microsensor readings.

[发布机构] University of Freiburg-IMTEK, Georges-Köhler-Allee 103, Freiburg; 79110, Germany^1;University of Oslo, P.O.Box 1072, Blindern, Oslo; 0316, Norway^2;Norwegian Radium Hospital, Ullernchauseen 70, Oslo; 0310, Norway^3

[效力级别] 力学 [学科分类] 力学，机械学

[关键词] Cellular respiration;Electrochemical characterizations;Electrochemical microsensors;Fluorescence signals;Metabolic parameters;Operation conditions;Sensor performance;Simultaneous monitoring