Automation of Silica Bead-based Nucleic Acid Extraction on a Centrifugal Lab-on-a-Disc Platform

[摘要] We describe a centrifugal microfluidic 'Lab-on-a-Disc' (LoaD) technology for DNA purification towards eventual integration into a Sample-to-Answer platform for detection of the pathogen Escherichia coli O157:H7 from food samples. For this application, we use a novel microfluidic architecture which combines 'event-triggered' dissolvable film (DF) valves with a reaction chamber gated by a centrifugo-pneumatic siphon valve (CPSV). This architecture permits comprehensive flow control by simple changes in the speed of the platform innate spindle motor. Even before method optimisation, characterisation by DNA fluorescence reveals an extraction efficiency of 58%, which is close to commercial spin columns.

[发布机构] School of Physical Sciences, National Centre for Sensor Research, Dublin City University, Glasnevin, Dublin, Ireland^1

[效力级别] 力学 [学科分类] 力学，机械学

[关键词] Acid extraction;Centrifugal microfluidics;DNA fluorescence;DNA purification;Escherichia coli O157:H7;Event-triggered;Extraction efficiencies;Reaction chambers