The principle and application of new PCR Technologies

[摘要] Polymerase chain reaction (PCR) is essentially a selective DNA amplification technique commonlyapplied for genetic testing and molecular diagnosis because of its high specificity and sensitivity.PCR technologies as the key of molecular biology, has realized that the qualitative detection of absolute quantitative has been changed. It has produced a variety of new PCR technologies, such as extreme PCR, photonic PCR, o-Amplification at lower denaturation temperature PCR, nanoparticle PCR and so on. In this paper, the principle and application of PCR technologies are reviewed, and its development is prospected too.

[发布机构] Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin; 150000, China^1

[关键词] Denaturation temperatures;DNA amplification;Genetic testing;High specificity;Molecular diagnosis;PCR technology