Extraction and determination of total flavonoids in jujube by alcohol extraction

[摘要] Jujube is a ripe fruit of Rhamnaceae. Its main active component is flavonoids, so the extraction and determination of total flavonoids in jujube will help to develop and utilize the medicinal value of jujube. In this study, the total flavonoids were extracted from jujube by alcohol extraction method. Through single factor investigation and orthogonal test, it was found that the total flavonoids content in jujube was the highest under the condition of 70°C, material ratio of 1:40, and extraction of 30 min by 70% ethanol. The content of total flavonoids in the extract of jujube was 1.57% at the wavelength of 510 nm by UV and rutin as the standard. The method was evaluated by methodological study, and it was determined that this method could be used as the detection of total flavonoids in jujube extraction.

[发布机构] Research Center on Life Science and Environmental Science, Harbin University of Commerce, Harbin; 150076, China^1;Engineering Research Center of Natural Anticancer Drugs, Ministry of Education, Harbin; 150076, China^2

[关键词] Active components;Extraction method;Medicinal values;Methodological studies;Orthogonal test;Total flavonoids