Study on Extraction, Isolation and Structure Identification of the Chemical Constituents of Hippeastrum vittatum

[摘要] The MTT assay results show that the four parts extraction of amaryllis leaves significantly and dose-dependently inhabit HepG2 cellular proliferation. After Separate and purify the compounds in n-butanol section by multiplex chromatography, two compounds were acquired. According to the date of 1H-NMR, 13C-NMR, MS and other spectroscopic data along with the physical and chemical properties, two compounds structure are identified, namely 2S-7,3'-dihydroxy-4'-methoxy-flavan and (2S)-7-hydroxy-3",4'-dimethoxy-flavan. This two compounds is isolated from Hippeastrum.

[发布机构] Life Sci. and Environ. Science Research Center of Harbin University of Commerce; Heilongjiang Harbin, 150076, China^1;Natl. Ministry of Education Anti-Tumor Natural Drug Engineering Research Center; Heilongjiang Harbin, 150076, China^2

[关键词] 13C NMR;Cellular proliferations;Chemical constituents;MTT assays;N-butanol;Physical and chemical properties;Spectroscopic data;Structure identification