Antiviral effects of Curcuma longa L. against dengue virus in vitro and in vivo

[摘要] Dengue is the most common infective disease caused by dengue virus (DENV) and endemic diseases in tropical and subtropical areas. Until now, there is no specific antiviral for dengue infection. It is known that viral load is related to disease severity. Curcuma longa L. (turmeric) with curcumin as major active compound has been identified for its antiviral effect. This study to determine antiviral effect of C. longa extract on DENV-2 in vitro and in vivo along with its toxicity in liver and kidney of ddY mice. Antiviral activity (IC50) and toxicity (CC50) in vitro was examined on Huh7it-1 cells by focus assay and a MTT assay, respectively. To determine the selectivity index (SI), we used CC50and IC50value. The safe doses obtained were used for toxicity tests of liver and kidney with histopathological and biochemical observations. The C. longa extracts was given orally with dose of 0.147 mg/mL for each mice at 2 hours after injected with DENV-2 infected Huh7it-1 cells. Serum was collected from intraorbital at 6 hours and 24 hours after infection and focus assay was used to determine viral load. In this study, the acquired value of IC50was 17,91 μg/mL whereas the value of CC50was 85,4 μg/mL. The value of SI of C. longa was 4.8. In vivo, we found that C. longa remarkable reduced of viral load after 24 hour. Histopathological examination showed no specific abnormalities in liver and kidney. There was no significant increase in levels of SGPT, SGOT, urea, and creatinine. From this study it can be concluded that C. longa could potentially be used as antiviral against DENV with low cytotoxicity and effective inhibition.

[发布机构] Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Dr Cipto Mangun Kusumo Hospital, JalanPegangsaanTimur no 16, Jakarta; 10320, Indonesia^1;Faculty of Medicine, Universitas Indonesia, Dr Cipto Mangun Kusumo Hospital, Jl. Salemba no 6, Jakarta, Indonesia^2

[效力级别] 化学 [学科分类] 生物科学（综合）

[关键词] Active compounds;Anti-viral effects;Antiviral activities;Disease severity;Histopathological examinations;Low cytotoxicities;Selectivity index;Subtropical area