Antiviral Effect of Sub Fraction Cassia alata Leaves Extract to Dengue Virus Serotype-2 strain New Guinea C in Human Cell Line Huh-7 it-1

[摘要] Dengue virus (DENV) is one of the most common viral infections found Indonesia and tropical regions, and no specific antiviral for DENV. Indonesia has several of herbal medicine that were not explored of their potency as antiviral DENV. This study was done to evaluate the activity and toxicity of 4 derived fractions: Hexane (CA1), ethyl acetate (CA2), buthanol (CA3 ) and water (CA4) of Cassia alata leaf extract (CA) as an antiviral drug to DENV. The DENV was treated with various concentration of extract and added to Huh-7 it-1. The decrease of virus titer was determined by Focus assay. The toxicity of extract was measured by MTT assay. In our previous study, we found that CA on Huh-7 cells showed IC50, CC50and SI values of 50, CC50and SI of

[发布机构] Doctoral Programme in Biomedical Science, Faculty of Medicine, University of Indonesia, Jl. Salemba Raya 6, Jakarta; 10430, Indonesia^1;Research Centre for Chemistry LIPI, Kompleks Puspiptek, Serpong; 15416, Indonesia^2;Departement of Microbiology Faculty of Medicine, Universitas Indonesia, Cipto Mangukusumo Hospital, Jalan Pengangsaan Timur No. 16, Jakarta; 10320, Indonesia^3;Department of Pharmacology and Therapeutics, Faculty of Medicine, RSCM Universitas Indonesia, Jl. Salemba Raya 6, Jakarta; 10430, Indonesia^4

[效力级别] 化学 [学科分类] 生物科学（综合）

[关键词] Anti-viral drugs;Anti-viral effects;Antiviral activities;Herbal medicines;Human cell lines;Inhibition mechanisms;Tropical regions;Viral infections