Chemical structure, comparison antioxidant capacity and separation antioxidant of hen, duck and quail egg white protein hydrolysate
[摘要] Amino acid linkages as proteins are nutritional substance which important for diet intake. Purification protein procesing undergo heating procedure process followed by additional of proteolytic enzymes or acid had been resulting in protein hydrolysates. A protein hydrolysate describe as many free amino acids bound together through a complex mixture of peptides. Egg white protein hydrolysates is one of subject interested to study for human health or industry product. The objectives of the research are to determine and identification the antioxidant derived from egg white hydrolysate protein. Identification of chemical structure of albumen and albumen protein hydrolysate was examine using IR Spectrophotometry. While comparison of antioxidant capacity and antioxidant separation egg albumen was also investigate using FTIR method (Fourier Transform Infrared Spectroscopy). Hen, duck and quail albumen egg white and on hydrolisate form were used as research materials. The results were showing that different time and enzyme of hydrolysis were not influence at secondary structure of hydrolysate albumen protein. Phytochemical content such as alcohol and hydroxyl compound which have potential as functional group of antioxidant were detected in all of the samples. Their results of radical scavenging activities samples hydrolyzed by pepsin were respectively 89.40%, 50.25% and 85.13%. Whereas the radical scavenging activities of hydrolysates hydrolyzed by papain were 72.85%, 61% and 76.45% respectively.
[发布日期] [发布机构] Faculty of Agriculture and Animal Sciene, State Islamic University of Sultan Syarif Kasim Riau, Indonesia^1;Live Stock Product Processing, Huazhong Agricultural University, Wuhan, China^2;Faculty of Animal Agricultural Sciences, Diponegoro University, Semarang, Indonesia^3
[效力级别] 生物科学 [学科分类]
[关键词] Antioxidant capacity;Eggs white hydrolisates;FTIR;IR spectrophotometry;Protein hydrolysate;Proteolytic enzyme;Radical scavenging activity;Secondary structures [时效性]
