Improvement of lipid yield from microalgae Spirulina platensis using ultrasound assisted osmotic shock extraction method

[摘要] Microalgae Spirulina sp. has been identified as potential source of natural food supplement and food colorant. The high water content of microalgae (70-90%) causes an obstacle in biomass dehydration which requires large amounts of energy, eventually damaging the lipid in the microalgae. Therefore, the lipid must be extracted by using a suitable method which complies to wet biomass conditions. One of the methods is applying osmotic shock. This study was aimed to investigate the influence of osmotic agent (NaCl) concentration (10-30%) and extraction time (20-50 min) on yield of lipid and also to determine the optimal conditions in the extraction process through response surface methodology. The extraction was conducted at a temperature of 40°C under ultrasound frequency of 40 kHz. The result showed that the optimum yield lipid obtained was 6.39% in 16.98% NaCl concentration for 36 minutes 10 seconds.

[发布机构] Department of Chemical Engineering Faculty of Engineering, Diponegoro University, Jl. Prof Soedarto, SH Tembalang, Semarang; 50275, Indonesia^1;Master Program of Environmental Science, School of Postgraduate Studies, Diponegoro University, Jl. Imam Bardjo No 3-5, Semarang, Indonesia^2

[效力级别] 生物科学

[关键词] Extraction process;Microalga;NaCl concentration;Optimal conditions;Osmotic shocks;Response surface methodology;Spirulina platensis;Ultrasound frequency