A novel colloidal gold labeled antigen for the detection of Deoxynivalenol using an immunochromatographic assay method

[摘要] In this paper, an immunochromatographic assay card was developed for the detection of DON in feed and cereals using a novel colloidal gold labeling method. For the colloidal gold immunochromatographic rapid detection (GICD) card, a monoclonal antibody DON-mAb and a goat anti-chicken IgY were drawn on NC membrane as the test line (T line) and the control line (C line) respectively. A gold labeled DON-CBSA conjugate and a gold labeled chicken IgY were sprayed onto the conjugate pad. The GICD card has cut-off levels of 50ng/mL for DON, which is invulnerable to matrix interference, and applicable to a wide range of samples. The GICD detecting results of feed and grain samples were compared with the results of ELISA testing, which showed good consistency.

[发布机构] School of Life Science, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi; 330013, China^1

[效力级别] 生态环境科学

[关键词] Colloidal gold;Control lines;Deoxynivalenol;Immunochromatographic;Immunochromatographic assays;Matrix interference;Rapid detection;Test-lines