Development of ochratoxin a in cereal by chemiluminescence enzyme immunoassay

[摘要] A rapid, simple and sensitive chemiluminescence enzyme immunoassay method (CLEIA) was established to detect ochratoxin A (OTA) in cereal. Optimal conditions including antibody dilution ratio and enzyme conjugate, ionic strength, pH value and organic solvent. Established indirect competition inhibition curve to determine the linear working range, detection limit and recovery rate. Results: The 50% inhibitory concentration and the detection limit of the CLEIA were78.8pg/mL and 14.86 pg/mL, respectively, with a linear range of 0.015-0.4ng/mL. At 1∼4μpg/kg fortified levels in wheat, mean recoveries ranged from 67.47% to100.35%.

[发布机构] School of Life Science, Jiangxi Science and Technology Normal University, Nanchang, Jiangxi; 330013, China^1

[效力级别] 生态环境科学

[关键词] Chemiluminescence enzyme immunoassay;Detection limits;Dilution ratio;Fortified level;Inhibitory concentration;Linear range;Optimal conditions;Recovery rate