Use of corn steep liquor as an economical nitrogen source for biosuccinic acid production by Actinobacillus succinogenes

[摘要] Expensive raw materials are the driving force that leads to the shifting of the petroleum-based succinic acid production into bio-based succinic acid production by microorganisms. Cost of fermentation medium is among the main factors contributing to the total production cost of bio-succinic acid. After carbon source, nitrogen source is the second largest component of the fermentation medium, the cost of which has been overlooked for the past years. The current study aimed at replacing yeast extract- a costly nitrogen source with corn steep liquor for economical production of bio-succinic acid by Actinobacillus succinogenes 130Z. In this study, a final succinic acid concentration of 20.6 g/L was obtained from the use of corn steep liquor as the nitrogen source, which was comparable with the use of yeast extract as the nitrogen source that had a final succinate concentration of 21.4 g/l. In terms of economical wise, corn steep liquor was priced at $ 200 /ton, which was one fifth of the cost of yeast extract at $ 1000 /ton. Therefore, corn steep liquor can be considered as a potential nitrogen source in biochemical industries instead of the costly yeast extract.

[发布机构] Chemical and Process Engineering Department, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor; 43600, Malaysia^1;Research Centre for Sustainable Process Technology (CESPRO), Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, Bangi, Selangor; 43600, Malaysia^2;School of Science, Monash University, Jalan Lagoon Selatan, Bandar Sunway, Selangor Darul Ehsan; 46150, Malaysia^3

[效力级别] 化学 [学科分类] 生物科学（综合）

[关键词] Acid production;Actinobacillus succinogenes;Biochemical industries;Corn steep liquor;Economical production;Fermentation medium;Nitrogen sources;Total production cost