Optimization of ultrasound-assisted extraction of flavonoids compounds from Chenopodium hybridum L. stem with response surface methodology

[摘要] Ultrasound-assisted extraction (UAE) of flavonoids compounds (FC) from the stem of Chenopodium hybridum L.(C.hybridum L.) was investigated in this paper. Significant technological parameters were screened and optimized by using Plackett-Burman (PB) design, Steepest ascent method and Box-Behnken (BB) design, respectively. A mathematical model with high correlation coefficient (R2=0.9896) was developed and showed good consistency between the experimental and predicted values. The optimum conditions for UAE were obtained by res- ponse surface methodology (RSM) as follows: volumn fraction of ethanol 76.62 %, extractive temperature 78.69°C, and liquid to solid ratio 58.43 for 30 min. Under these conditions, total flavo- noid content (TFC) of 9.4701 mg RE/100g were gained and it was closely related with predi- cted value (9.4640 mg RE/100g) and indicated the suitability of the developed model.

[发布机构] College of Chemistry and Chemical Engineering, Longdong University, Qingyang, China^1;College of Life Science and Technology, Longdong University, Qingyang, China^2

[效力级别] 生物科学

[关键词] Correlation coefficient;Flavonoids compounds;Liquid to solid ratio;Plackett -burman design;Response surface methodology;Surface methodologies;Technological parameters;Ultrasound-assisted extraction