An indole alkaloid produced by Indonesian's marine sponge Raspailia ramosa as an inhibitory of the panc-1 cell adapted to nutrient starvation

[摘要] The bioactivity from Indonesian's Marine Sponge Raspailia ramosa has been done on July 2018. Bioactive compound isolated based on bioassay-guided separation with several steps of chromatography. The active metabolite compound of Raspailia ramosa has cytotoxic activity against PANC-1 cell with IC50 = 10g/mL. Based on NMR spectrum shows that the active compound has molecular formula C16H17N4Br determined by LCMS-ESI with molecular weight [M+H]+345.23 m/z. The FTIR spectrum shows that metabolic metabolites have NH secondary amines 3435.56 cm-1 with CN imine fingerprint area (1637.27 cm-1), CH methyl (2853.39 cm-1), CH methylene (2769.64 cm-1) indicate as indole alkaloid. The active metabolite compound of Raspailia ramosa has cytotoxic activity against PANC-1 cellwithIC50 = 10 g / mL. This compound has the potential activity for the drug model for commercial drug antimycin = 0.1 μg / mL.

[发布机构] Department of Marine Science, Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh; 23111, Indonesia^1;Marine Chemistry Laboratory, Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh; 23111, Indonesia^2;Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa; 903-0213, Japan^3

[效力级别] 农业科学

[关键词] Active metabolites;Bioactive compounds;Cytotoxic activities;Indole alkaloids;Molecular formula;Nutrient-starvation;Potential activities;Secondary amines