Short-term and long-term effects of copper on the performance and microorganisms in sequencing batch reactor

[摘要] The present work investigated the short-term and long-term impacts of copper ion (Cu2+) on the variation of microbial community structures and pollutant (carbon, nitrogen, and phosphorus) removal performance in sequencing batch reactor (SBR). The results showed that the short-term exposure (within 1 cycle) of 7 mg/L or higher concentration of Cu2+ decreased the removal efficiencies of dissolved organic carbon (DOC) and orthophosphate (PO4 3--P), as well as the ATP content and dehydrogenase activity. In the long-term exposure test (30 days), the addition of Cu2+ at 3 mg/L significantly compromised the removal performance of DOC, ammonia-nitrogen (NH4+-N) and PO4 3--P. Higher production of intracellular reactive oxygen species (ROS) was observed along with increasing activities of catalase (CAT) and superoxide dismutase (SOD). The increase of lactate dehydrogenase (LDH) release was detected at the end of the experiment, indicating the occurrence of membrane damage and cell leakage. The microbial diversity and community structure changed with time, which would also be another important factor that affects the pollutant removal performance in SBR.

[发布机构] State Key Laboratory of Eco-hydraulics in Northwest Arid Region of China, Xian University of Technology, Xian, Shaanxi; 710048, China^1;Department of Civil and Environmental Engineering, North-eastern University, 360 Huntington Avenue, Boston; MA; 02115, United States^2;State Key Laboratory of Loess and Quaternary Geology, Institute of Earth Environment, Chinese Academy of Science, Xian, Shaanxi; 710075, China^3

[效力级别] 生态环境科学 [学科分类] 环境科学（综合）

[关键词] Dehydrogenase activity;Dissolved organic carbon;Long term exposure test;Microbial community structures;Pollutant removal performance;Reactive oxygen species;Sequencing batch reactors;Superoxide dismutases