Binding of the Bi (III) Complex of Naringin withβ-Cyclodextrin/Calf Thymus DNA: Absorption and Fluorescence Characteristics

[摘要] Naringin-Bi (III) complex (Narb) was prepared and analysed by UV-Visible absorption and fluorescence measurements. The inclusion complex of Narb withβ-Cyclodextrin (β-CD) was characterized by the UV-Visible absorption, Infrared, scanning dlectron microscopic, and X-ray diffractometric techniques. The stoichiometry of the inclusion complex of Narb withβ-CD was 1 : 1 with a binding constant of 5.18 × 102mol−1dm3. The interaction of Narb with Calf Thymus DNA (ctDNA) was investigated in the presence and the absence ofβ-CD.The binding constants for the interaction of Narb with ctDNA in the absence and the presence ofβ-CD were1.29 × 105mol−1dm3and 6.89 × 104mol−1dm3, respectively.The Stern-Volmer constants for the interaction of Narb with ctDNA in the absence and the presence ofβ-CD were1.25 × 104mol−1dm3and 5.10 × 103mol−1dm3, respectively.The lowering of the binding affinity and theKsvwere observed for the interaction of Narb with ctDNA in the presence ofβ-CD.

[学科分类] 光谱学