Efficacy of Essential Oils ofThymus vulgarisandOriganum vulgareonEchinococcus granulosus

[摘要] The aim of the present work was to determine thein vitroeffect ofT. vulgarisandO. vulgareessential oils againstE. granulosusprotoscoleces and cysts. Essential oils were added to the medium resulting in thymol final concentrations of 10 μg/mL. The essential oils had a time-dependent effect provoking the complete loss of protoscolex viability after 72 days of postincubation. The results were confirmed at the ultrastructure level. Loss of infectivity in protoscoleces incubated withO. vulgareafter 60 days was observed. On the other hand, the weight of cysts recorded in mice inoculated withT. vulgaristreated protoscoleces was significantly lower than that obtained in control group. Gamma-glutamyl-transpeptidase activity was readily detected in the culture supernatant of protoscoleces treated either with the essential oils or thymol.T. vulgarisandO. vulgareessential oils and thymol can induce cell apoptosis of protoscoleces after short incubation times. The efficacy ofT. vulgarisandO. vulgareessential oils was also demonstratedin vitroonE. granulosusmurine cysts. Our data suggest that essential oils ofT. vulgarisandO. vulgarehave anthelmintic effect against protoscoleces and cysts ofE. granulosus.

[学科分类] 传染病学