Identification of Sphingomyelinase on the Surface ofChlamydia pneumoniae: Possible Role in the Entry into Its Host Cells

[摘要] We have recently suggested a novel mechanism, autoendocytosis, for the entry of certain microbes into their hosts, with a key role played by the sphingomyelinase-catalyzed topical conversion of sphingomyelin to ceramide, the differences in the biophysical properties of these two lipids providing the driving force. The only requirement for such microbes to utilize this mechanism is that they should have a catalytically active SMase on their outer surface while the target cells should expose sphingomyelin in the external leaflet of their plasma membrane. In pursuit of possible microbial candidates, which could utilize this putative mechanism, we conducted a sequence similarity search for SMase. Because of the intriguing cellular and biochemical characteristics of the poorly understood entry ofChlamydiainto its host cells these microbes were of particular interest. SMase activity was measuredin vitrofrom isolatedC. pneumoniaeelementary bodies (EB) and in the lysate fromE. colicells transfected with a plasmid expressing CPn0300 protein having sequence similarity to SMase. Finally, pretreatment of host cells with exogenous SMase resulting in loss plasma membrane sphingomyelin attenuated attachment of EB.

[学科分类] 传染病学