In VitroCestocidal Activity of Thymol onMesocestoides cortiTetrathyridia and Adult Worms

[摘要] Nothing is known about the possible effect of thymol or other compounds of essential oils against the adult worms of cestodes. The aim of the present work was to determinein vitrocestodicidal activity of thymol againstMesocestoides cortiadult worms. Moreover, thein vitroeffect on tetrathyridia was also demonstrated. Tetrathyridia exposed to different concentrations of thymol showed a concentration and time-dependent effect. At lower concentrations, the main change observed was mainly in morphology, with larvae exhibiting an elongation of the body. When tetrathyridia were exposed to higher concentrations, increased surface alterations and damage were detected. The body appeared elongated and flattened, and a complete loss of morphology and microtriches was observed. Thymol was able to killM. cortitetrathyridia, since following inoculation of treated parasites in mice no parasites could be recovered. The effect onM. cortiadult worms was dose and time-dependent. Changes in motility coincide with the tissue damage were observed at the structural and ultrastructural level. Thymol caused severe damages to both developmental stages analyzed. Damages were more significant in fully segmented worms. The data reported in this paper demonstrate a clearin vitroeffect of thymol againstM. cortitetrathyridia and adult worms.

[学科分类] 传染病学