Molecular and Clinical Characterization ofGiardia duodenalisInfection in Preschool Children from Lisbon, Portugal

[摘要] Giardia duodenalisis the most prevalent intestinal protozoan infection especially in children. In Portugal scarce data are available relative to this infection in preschoolers. The present study was conducted from April to July 2009 in public preschools in Lisbon enrolling 316 children. Stool examination was performed through microscopy. Molecular analysis was conducted in all positive samples forG. duodenalisin order to determine the assemblage and subassemblage of this parasite. Eight of the preschoolers studied children (2.5%, 8/316) were infected withG. duodenalis. Additionally the brother of one of the infected children was also infected. Genotyping analysis targetingssu-rRNAandβ-giardinloci revealed six infections with assemblage A and 3 with assemblage B. Sub-assemblage determination was possible in four of the samples, with three A2 and one A3. The limited number of cases precluded an association of a determined symptom with an assemblage. The data presented here show the relevance of consideringG. duodenalisanalysis in children with intestinal complaints even in developed countries.

[学科分类] 神经科学