In VitroAntiparasitic and Apoptotic Effects of Antimony Sulfide Nanoparticles onLeishmania infantum

[摘要] Visceral leishmaniasis is one of the most important sever diseases in tropical and subtropical countries. In the present study the effects ofantimony sulfide nanoparticles onLeishmania infantum in vitrowere evaluated. Antimony sulfide NPs (Sb2S5) were synthesized bybiological method fromSerratia marcescensbacteria. Then the cytotoxicity effects of different concentrations (5, 10, 25, 50, and 100 μg/mL) of this nanoparticle were assessed on promastigote and amastigote stages ofL. infantum. MTT method was used for verification results of promastigote assay. Finally, the percentages of apoptotic,necrotic, and viable cells were determined by flow cytometry. The results indicated the positive effectiveness of antimony sulfide NPs on proliferation of promastigoteform. The IC50(50% inhibitory concentration) of antimony sulfide NPs on promastigotes was calculated 50 μg/mL. The cytotoxicity effect was dose-dependent means by increasing the concentration of antimony sulfide NPs, the cytotoxicity curve was raised and the viability curve of the parasite dropped simultaneously. Moreover, the IC50of antimony sulfide NPs on amastigote stage was calculated 25 μg/mL. On the other hand, however, antimonysulfide NPs have a low cytotoxicity effect on uninfected macrophages but it caninduce apoptosis in promastigote stage at 3 of 4 concentrations.

[学科分类] 神经科学