Enterocytozoon bieneusiIdentification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo

[摘要] Objective. To determine the prevalence and the genotypes ofEnterocytozoon bieneusiin stool specimens from HIV patients.Methods. This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012. Detection of microsporidia includingE. bieneusiandE. intestinaliswas performedin 242 HIV-infected patients. Typing was based on DNA polymorphism of the ribosomal DNA ITS region ofE. bieneusi. PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages. The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard.Results. Out of 242 HIV-infected patients, using the real-time PCR, the prevalence ofE. bieneusiwas 7.9% (n=19) among the 19E. bieneusi, one was coinfected withE. intestinalis. In 19E. bieneusipersons using PCR-RFLP method, 5 type I strains ofE. bieneusi(26.3%) and 5 type IV strains ofE. bieneusi(26.3%) were identified. The sensitivity of IFI-AcM was poor as estimated 42.1%.Conclusion. Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains.

[学科分类] 神经科学