Changes in T-Cell and Monocyte PhenotypesIn VitrobySchistosoma mansoniAntigens in Cutaneous Leishmaniasis Patients

[摘要] High levels of proinflammatory cytokines such as IFN-γand TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated thatSchistosoma mansoniantigens downmodulate thein vitrocytokine response in CL. In the current study we evaluated whetherS. mansoniantigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured withL. braziliensisantigen in the presence or absence of theS. mansoniantigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14+CD16++) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14++CD16-) and intermediate (CD14++CD16+) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4+T cells and they also expanded the frequency of CD4+CD25highFoxp3+T cells. Taken together, we show thatS. mansoniantigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes.

[学科分类] 神经科学