Application of RFLP-PCR-Based Identification for Sand FlySurveillance in an Area Endemic for Kala-Azar in Mymensingh, Bangladesh

[摘要] Mymensingh is the most endemic district for kala-azar in Bangladesh.Phlebotomus argentipesremains the only known vector although a number of sand fly species are prevalent in this area. Genotyping of sand flies distributed in a VL endemic area was developed by a PCR and restriction-fragment-length polymorphism (RFLP) of 18S rRNA gene of sand fly species. Using the RFLP-PCR analysis withAfaI andHinfI restriction enzymes,P. argentipes, P. papatasi, andSergentomyiaspecies could be identified. Among 1,055 female sand flies successfully analyzed for the species identification individually, 64.4% flies was classified asSergentomyiaspecies, whereas 35.6% was identified asP. argentipesand noP. papatasiwas found. Although infection ofLeishmaniawithin the sand flies was individually examined targeting leishmanial minicircle DNA, none of the 1,055 sand flies examined were positive forLeishmaniainfection. The RFLP-PCR could be useful tools for taxonomic identification andLeishmaniainfection monitoring in endemic areas of Bangladesh.

[学科分类] 神经科学