Selection of Housekeeping Genes for Transgene Expression Analysis inEucommia ulmoidesOliver Using Real-Time RT-PCR

[摘要] In order to select appropriate housekeeping genes for accurate calibration of experimental variations in real-time (RT-) PCR results in transgene expression analysis, particularly with respect to the influence of transgene on stability of endogenous housekeeping gene expression in transgenic plants, we outline a reliable strategy to identify the optimal housekeeping genes from a set of candidates by combining statistical analyses of their (RT-) PCR amplification efficiency, gene expression stability, and transgene influences. We used the strategy to select two genes,ACTαandEF1α, from 10 candidate housekeeping genes, as the optimal housekeeping genes to evaluate transgenicEucommia ulmoidesOliver root lines overexpressingIPPIorFPPS1genes, which are involved in isoprenoid biosynthesis.

[学科分类] 植物学