Antibacterial Activity of the Extracts Obtained fromRosmarinus officinalis,Origanum majorana, andTrigonella foenum-graecumon Highly Drug-Resistant Gram Negative Bacilli

[摘要] Our aim was to determine the antimicrobial activity of three selected plants (Rosmarinus officinalis,Origanum majorana, andTrigonella foenum-graecum) against Extended Spectrum Beta Lactamase (ESBL)—producingEscherichia coliandKlebsiella pneumoniae— and to identify the specific plant fraction responsible for the antimicrobial activity. The plants were extracted with ethanol to yield the crude extract which was further subfractionated by different solvents to obtain the petroleum ether, the dichloromethane, the ethyl acetate, and the aqueous fractions. The Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) were determined using broth microdilution. The MICs ranged between 1.25 and 80 μg/μl. The majority of these microorganisms were inhibited by 80 and 40 μg/μlof the crude extracts. The petroleum ether fraction ofOriganum majoranasignificantly inhibited 94% of the tested strains. Ethyl acetate extracts of all selected plants exhibited relatively low MICs and could be therefore described as strong antibacterial.

[学科分类] 植物学