Emergence of Hepatitis B Virus Genotype F in Aligarh Region of North India

[摘要] Introduction. HBV genotypes and subtypes are useful clinical and epidemiological markers. In this study prevalent HBV genotypes were assessed in relation to serological profile and clinical status.Material & Methods. 107 cases of HBV were genotyped. Detailed clinical history was elicited from them. HBsAg, HBeAg, anti-HBs, anti-HBe, and anti-HBc-IgM were assessed. HBV genotyping was performed using Kirschberg's type specific primers (TSP-PCR), heminested PCR, and Naito's monoplex PCR. Nucleotide sequencing was performed.Results. A total of 97 (91%) were genotyped following the methods of Kirschberg et al./Naito et al.Genotype D was by far the most prevalent genotype 91 (85.04%) in this region. A surprising finding was the detection of genotype F in 5 (4.67%) of our patients. Genotype A strangely was observed only in one case. In 85.7% genotype D was associated with moderate to severe liver disease, 43.9% HBeAg, and 18.7% anti-HBc-IgM positivity. Majority of genotype F (80%) was seen in mild to moderate liver disease. It was strongly associated with HBeAg 60% and 20%anti-HBc-IgM positivity.Conclusion.Emergence of genotype F in India merits further study regarding its clinical implications and treatment modalities.Knowledge about HBV genotypes can direct a clinician towards more informed management of HBV patients.

[学科分类] 微生物学和免疫学