Capillary Electrophoresis Single-Strand Conformational Polymorphisms as a Method to Differentiate Algal Species

[摘要] Capillary electrophoresis single-strand conformational polymorphism (CE-SSCP) was explored as a fast and inexpensive method to differentiate both prokaryotic (blue-green) and eukaryotic (green and brown) algae. A selection of two blue-green algae (Nostoc muscorumandAnabaena inaequalis), five green algae (Chlorella vulgaris, Oedogonium foveolatum, Mougeotiasp.,Scenedesmus quadricauda, andUlothrix fimbriata), and one brown algae (Ectocarpussp.) were examined and CE-SSCP electropherogram “fingerprints” were compared to each other for two variable regions of either the 16S or 18S rDNA gene. The electropherogram patterns were remarkably stable and consistent for each particular species. The patterns were unique to each species, although some common features were observed between the different types of algae. CE-SSCP could be a useful method for monitoring changes in an algae species over time as potential shifts in species occurred.

[学科分类] 分析化学