DNA binding and photo-induced DNA cleavage activity of Elsinochrome A in visible light

[摘要] The interaction of Elsinochrome A (EA) with calf thymus DNA (CT-DNA) has been investigated by UV-vis spectra and fluorescence spectra. The results show that EA can bind with CT-DNA and binding sites are destroyed after irradiation by visible light, which indicates that EA is a promising candidate for photodynamic therapy. In addition, the binding mechanism is studied using fluorescence quenching test and ethidium bromide (EB) replace assay experiments. The results suggest that EA and CT-DNA are binding with a strong force and the major binding mode of EA with DNA could be the electrostatic binding.

[学科分类] 光谱学