Prooxidative and fluorescence properties of paracetamol during interactions with mitochondria

[摘要] This study was carried out to investigate isolated liver mitochondrial functions after paracetamol administration by monitoring of liver mitochondrial fluorescence properties as well as prooxidative properties of paracetamol. Paracetamol was administered to rat (in subtoxic 500 mg·kg−1dose) invivo. The effect of this dose was compared with the subtoxic and toxic dose of paracetamol added to mitochondria invitro(1 and 1.5) mg paracetamol/mg mitochondrial protein. Subtoxic dose of paracetamol invitrodid not change mitochondrial fluorescence, but it significantly decreased mitochondrial fluorescence invivoin comparison with control mitochondrial group. Toxic dose of paracetamol invitrosignificantly decreased mitochondrial fluorescence. The enzymatic activity of superoxide dismutase (SOD) significantly decreased after paracetamol administration invitroand invivo. While both activities of glutathione peroxidase (GPx) and glutathione reductase (GR) significantly increased in dependence upon paracetamol doses. Our experiment showed, that paracetamol participates in formation of free radicals and confirms previous studies, in which paracetamol administration caused elevation of antioxidative enzymes activities in dependence on dose, which is considered therapeutically as subtoxic and toxic.

[学科分类] 光谱学