Dominant effect of ethanol in thermal destabilization of bovine serum albumin in the presence of sucrose

[摘要] The thermal melting of bovine serum albumin (BSA) in the presence of excipients like ethanol and sucrose was studied by circular dichroism spectroscopy at physiological pH in phosphate buffered saline. Calculated apparentTmvalues were used to assess the thermal stability using two state fitted experimental curves. 0.5 M sucrose stabilized the BSA indicated by the increase inTmof ∼8°C when compared to theTmof the same solution measured in the absence of sucrose. Conversely, in the presence of varying concentrations of ethanol (2–20%), the protein was destabilized by a decrease of ∽3–10°C ofTm. In the binary mixture of sucrose and ethanol, theTmvalues showed that ethanol dominantly destabilized BSA in the presence of sucrose, possibly by weakening the hydrophobic interactions in the protein.

[学科分类] 光谱学