Effect ofAgaricus blazeiMurill on the Pulmonary Tissue of Animals with Streptozotocin-Induced Diabetes

[摘要] The present study was designed to evaluate the oxidative stress as well as the therapeutic effect ofAgaricus blazeiMuril (A. Blazei) in rats with streptozotocin-induced diabetes. We used 25 Wistar rats, and DM was induced by injecting streptozotocin (70 mg/Kg i.p.).Agaricus blazeiMuril was administered daily starting 40 days after disease onset.A. Blazeiwas tested as an aqueous extract for its phytochemical composition, and its antioxidant activity in vitro was also evaluated. Lipoperoxidation (LPO), and superoxide dismutase (SOD), catalase, and glutathione peroxidase activities were measured in the pulmonary tissue, as well as the presence of inducible nitric oxide synthase (iNOS), through immunohistochemistry. An anatomopathologic study was also performed. Phytochemical screening ofA. Blazeidetected the presence of alkaloids and saponins. The extract exhibited a significant antioxidant activity in the DPPH-scavenging and the hipoxanthine/xanthine oxidase assays. Pulmonary LPO increased in diabetic animals (0.43±0.09;P<.001) as compared to the control group (0.18±0.02), followed by a reduction in theA. Blazei-treated group (0.33±0.04;P<.05). iNOS was found increased in the lung in diabetic rats and reduced in theA. Blazei-treated group. The pulmonary tissue in diabetic rats showed oxidative alterations related to the streptozotocin treatment. TheA. Blazeitreatment effectively reduced the oxidative stress and contributed to tissue recovery.

[学科分类] 内分泌与代谢学