Mutational and Bioinformatic Analysis ofHaloarchaeal Lipobox-Containing Proteins

[摘要] A conserved lipid-modified cysteine found in a protein motif commonly referred to as a lipobox mediates the membrane anchoring of a subset of proteins transported across the bacterial cytoplasmic membrane via the Sec pathway. Sequenced haloarchaeal genomes encode many putative lipoproteins and recent studies have confirmed the importance of the conserved lipobox cysteine for signal peptide processing of three lipobox-containing proteins in the model archaeonHaloferax volcanii. Wehave extended thesein vivoanalyses to additionalHfx. volcaniisubstrates, supporting our previousin silicopredictions and confirming the diversity of predictedHfx. volcaniilipoproteins. Moreover, usingextensive comparative secretome analyses, we identified genes encodining putative lipoproteins across a wide range of archaeal species. While ourin silicoanalyses, supported byin vivodata, indicate that most haloarchaeal lipoproteins are Tat substrates, these analyses also predict that many crenarchaeal species lack lipoproteins altogether and that other archaea, such as nonhalophilic euryarchaeal species, transport lipoproteins via the Sec pathway. To facilitate the identification of genes that encode potential haloarchaeal Tat-lipoproteins, we have developed TatLipo, a bioinformatic tool designed to detect lipoboxesin haloarchaeal Tat signal peptides. Our results provide a strong foundation for future studies aimed at identifying components of the archaeal lipoprotein biogenesis pathway.

[学科分类] 微生物学和免疫学