Humidity-Dependent Bacterial Cells Functional Morphometry Investigations Using Atomic Force Microscope

[摘要] The effect of a relative humidity (RH) in a range of 93–65% on morphological and elastic properties ofBacillus cereusandEscherichia colicells was evaluated using atomic force microscopy. It is shown that gradual dehumidification of bacteria environment has no significant effect on cell dimensional features and considerably decreases them only at 65% RH. The increasing of the bacteria cell wall roughness and elasticity occurs at the same time. Observed changes indicate that morphological properties ofB. cereusare rather stable in wide range of relative humidity, whereasE. coliare more sensitive to drying, significantly increasing roughness and stiffness parameters at RH≤84% RH. It is discussed the dependence of the response features on differences in cell wall structure of gram-positive and gram-negative bacterial cells.

[学科分类] 微生物学和免疫学