Characterization of theEscherichia coliAntifungal Protein PPEBL21

[摘要] An antifungal protein isolated fromEscherichia coliBL21 (PPEBL21) and predicted to be alcohol dehydrogenase (ADH) was subjected to biological characterization. The PPEBL21, indeed, demonstrated propionaldehyde-specific ADH activity. The Km and Vmax of PPEBL21 were found to be 644.8μM and 1.2 U/mg, respectively. In-gel activity assay also showed that PPEBL21 was a propionaldehyde-specific ADH. The pI of PPEBL21 was observed to be 7.8. PPEBL21 was found to be stable up to a temperature of40∘Cwith optimum activity at pH 7.5. The decrease in pH decreased the activity of PPEBL21. These results suggested that PPEBL21 having alcohol dehydrogenase activity and stability at significantly high temperature might be an important lead antifungal molecule. Experiments were performed to identify the possible target of PPEBL21 in the pathogenA. fumigatus. Results revealed that PPEBL21 inhibited completely the expression of a 16 kDa protein inA. fumigatus. The 16 kDa protein ofA. fumigatustargeted by PPEBL21 was identified as a hypothetical protein by peptide mass fingerprinting. It is thus hypothesized that a 16 kDa factor is essentially required byA. fumigatusfor survival and its impaired synthesis due to treatment with PPEBL21 may lead to the death of pathogen.

[学科分类] 微生物学和免疫学