Transcriptional Regulation of thersbVPromoter Controlling Stress Responses to Ethanol, Carbon Limitation, and Phosphorous Limitation inBacillus subtilis

[摘要] TheσB-dependent promoter in front of thersbVgene ofBacillus subtilisis induced~5-fold in response to (1) the addition of 4% ethanol, (2) carbon starvation, and (3) phosphorous starvation.Binding sites for the global carbon and nitrogen regulators, CcpA and TnrA, were mutated, and the consequences of their loss and that of CcpA or TnrA were studied usingrsbV-lacZfusions.These responses proved to be dependent on CcpA, TnrA, and their putative binding sites upstream of the promoter. Induction in response to glucose limitation was largely abolished by loss of CcpA or the upstream region, while induction in response to phosphorous limitation was largely abolished only by the upstream mutations. The results suggest that CcpA directly influences the carbon starvation response and that both proteins exert indirect effects on all three stress responses. The integrity of the DNA sequence is important for all three responses.

[学科分类] 微生物学和免疫学