Characterization of pPCP1 Plasmids inYersinia pestisStrains Isolated from the Former Soviet Union

[摘要] Complete sequences of 9.5-kb pPCP1 plasmids in threeYersinia pestisstrains from the former Soviet Union (FSU) were determined and compared with those of pPCP1 plasmids in three well-characterized, non-FSUY. pestisstrains (KIM, CO92, and 91001). Two of the FSU plasmids were from strains C2614 and C2944, isolated from plague foci in Russia, and one plasmid was from strain C790 from Kyrgyzstan.Sequence analyses identified four sequence types among the six plasmids.The pPCP1 plasmids in the FSU strains were most genetically related to the pPCP1 plasmid in the KIM strain and least related to the pPCP1 plasmid inY. pestis91001.The FSU strains generally had larger pPCP1 plasmid copy numbers compared to strain CO92.Expression of the plasmid'splagene was significantly (P≤.05) higher in strain C2944 than in strain CO92.Givenpla's role inY. pestisvirulence, this difference may have important implications for the strain's virulence.

[学科分类] 微生物学和免疫学