Production ofβ-Glucosidase from a Newly IsolatedAspergillusSpecies Using Response Surface Methodology

[摘要] A newly isolated fungusAspergillus nigerSOI017 was shown to be a good producer ofβ-glucosidase from all isolated fungal strains. Fermentation condition (pH, cellobiose concentration, yeast extract concentration, and ammonium sulfate concentration) was optimized for producing the enzyme in shake flask cultures. Response surface methodology was used to investigate the effects of 4 fermentation parameters (yeast extract concentration, cellobiose concentration, ammonium sulfate concentration, and pH) onβ-glucosidase enzyme production. Production ofβ-glucosidase was most sensitive to the culture medium, especially the nitrogen source yeast extract. The optimized medium for producing maximumβ-glucosidase specific activity consisted of 0.275% yeast extract, 1.125% cellobiose, and 2.6% ammonium sulfate at a pH value of 3.

[学科分类] 微生物学和免疫学