Characterization ofHalorubrum sfaxensesp. nov., a New Halophilic Archaeon Isolated from the Solar Saltern of Sfax in Tunisia

[摘要] An extremely halophilic archaeon, strain ETD6, was isolated from a marine solar saltern in Sfax, Tunisia. Analysis of the 16S rRNA gene sequence showed that the isolate was phylogenetically related to species of the genusHalorubrumamong the familyHalobacteriaceae, with a close relationship toHrr. xinjiangense(99.77% of identity). However, value for DNA-DNA hybridization between strain ETD6 andHrr.xinjiangensewere about 24.5%. The G+C content of the genomic DNA was 65.1 mol% (T(m)). Strain ETD6 grew in 15–35% (w/v) NaCl. The temperature and pH ranges for growth were 20–55°C and 6–9, respectively. Optimal growth occurred at 25% NaCl, 37°C, and pH 7.4. The results of the DNA hybridization againstHrr. xinjiangenseand physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain ETD6 from otherHrr.species. Therefore, strain ETD6 represents a novel species of the genusHalorubrum, for which the nameHrr. sfaxensesp. nov. is proposed. The Genbank EMBL-EBI accession number is GU724599.

[学科分类] 微生物学和免疫学