Gastrointestinal Bacterial and Methanogenic Archaea Diversity Dynamics Associated with Condensed Tannin-Containing Pine Bark Diet in Goats Using 16S rDNA Amplicon Pyrosequencing

[摘要] Eighteen Kiko-cross meat goats (n=6) were used to collect gastrointestinal (GI) bacteria and methanogenic archaea for diversity measures when fed condensed tannin-containing pine bark (PB). Three dietary treatments were tested: control diet (0% PB and 30% wheat straw (WS);0.17% condensed tannins (CT) dry matter (DM)); 15% PB and 15% WS (1.6% CT DM), and 30% PB and 0% WS (3.2% CT DM). A 16S rDNA bacterial tag-encoded FLX ampliconpyrosequencing technique was used to characterize and elucidate changes in GI bacteria and methanogenic archaea diversity among the diets.Proteobacteriawas the most dominant phylum ingoats with mean relative abundance values ranging from 39.7 (30% PB) to 46.5% (control) and47.1% (15% PB). Other phyla individually accounted for fewer than 25% of the relativeabundance observed. Predominant methanogens wereMethanobrevibacter(75, 72, and 49%),Methanosphaera(3.3, 2.3, and 3.4%), andMethanobacteriaceae(1.2, 0.6, and 0.7%) populationin control, 15, and 30% PB, respectively. Among methanogens,Methanobrevibacterwas linearly decreased (P=0.05) with increasing PB supplementation. These results indicate that feeding PBselectively altered bacteria and methanogenic archaeal populations in the GI tract of goats.

[学科分类] 微生物学和免疫学