An MFS Transporter-Like ORF from MDRAcinetobacter baumanniiAIIMS 7 Is Associated with Adherence and Biofilm Formation on Biotic/Abiotic Surface

[摘要] A major facilitator superfamily (MFS) transporter-like open reading frame (ORF) of 453 bp was identified in a pathogenic strainAcinetobacter baumanniiAIIMS 7, and its association with adherence and biofilm formation was investigated. Reverse transcription PCR (RT-PCR) showed differential expression in surface-attached biofilm cells than nonadherent cells.In vitrotranslation showed synthesis of a~17 kDa protein, further confirmed by cloning and heterologous expression inE. coliDH5α. Up to 2.1-, 3.1-, and 4.1- fold biofilm augmentation was observed on abiotic (polystyrene) and biotic (S. cerevisiae/HeLa) surface, respectively. Scanning electron microscopy (SEM) andgfp-tagged fluorescence microscopy revealed increased adherence to abiotic (glass) and biotic (S. cerevisiae) surface. Extracellular DNA(eDNA) was found significantly during active growth; due to probable involvement of the protein in DNA export, strong sequence homology with MFS transporter proteins, and presence of transmembrane helices. In summary, our findings show that the putative MFS transporter-like ORF (pmt) is associated with adherence, biofilm formation, and probable eDNA release inA. baumanniiAIIMS 7.

[学科分类] 微生物学和免疫学