New Spectrophotometric and Fluorimetric Methods for Determination of Fluoxetine in Pharmaceutical Formulations

[摘要] New simple and sensitive spectrophotometric and fluorimetricmethods have been developed and validated for the determination offluoxetine hydrochloride (FLX) in its pharmaceutical formulations. The spectrophotometric method was based on the reaction of FLXwith 1,2-naphthoquinone-4-sulphonate (NQS) in an alkaline medium(pH 11) to form an orange-colored product that was measured at 490nm. The fluorimetric method was based on the reaction of FLX with4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in an alkalinemedium (pH 8) to form a highly fluorescent product that wasmeasured at 545 nm after excitation at 490 nm. The variablesaffecting the reactions of FLX with both NQS and NBD-Cl werecarefully studied and optimized. The kinetics of the reactionswere investigated, and the reaction mechanisms were presented. Under the optimum reaction conditions, good linear relationshipswere found between the readings and the concentrations of FLX inthe ranges of 0.3–6 and 0.035–0.5 μgmL−1for thespectrophotometric and fluorimetric methods, respectively. Thelimits of detection were 0.1 and 0.01 μgmL−1for thespectrophotometric and fluorimetric methods, respectively. Bothmethods were successfully applied to the determination of FLX inits pharmaceutical formulations.

[学科分类] 分析化学