Similarity Evaluation of Different Origins and Species of Dendrobiums by GC-MS and FTIR Analysis of Polysaccharides

[摘要] GC-MS method combined with FTIR techniques by the analysis of polysaccharide was applied to evaluate the similarity between wild (W) and tissue-cultured (TC)Dendrobium huoshanense(DHS),Dendrobium officinale(DO), andDendrobium moniliforme(DM) as well as 3 wildDendrobiumspp.:Dendrobium henanense(DHN),Dendrobium loddigesii(DL), andDendrobium crepidatum(DC). Eight monosaccharides involving xylose, arabinose, rhamnose, glucose, mannose, fructose, galactose, and galacturonic acid were identified in the polysaccharide from eachDendrobiumsample while the contents of the monosugars varied remarkably across origins and species. Further similarity evaluation based on GC-MS data showed that thercorvalues of different origins of DHS, DO, and DM were 0.831, 0.865, and 0.884, respectively, while thercorvalues ranged from 0.475 to 0.837 across species. FTIR files of the polysaccharides revealed that the similarity coefficients between W and TC-DHS, DO, and DM were 88.7%, 86.8%, and 88.5%, respectively, in contrast to the similarity coefficients varying from 57.4% to 82.6% across species. These results suggested that the structures of polysaccharides between different origins of the investigatedDendrobiumsmight be higher than what we had supposed.

[学科分类] 分析化学