Experimental Design Optimization of a Sequential Injection Method for Promazine Assay in Bulk and Pharmaceutical Formulations

[摘要] Experimental design optimization approach was utilized to develop asequential injection analysis (SIA) method for promazine assay in bulk andpharmaceutical formulations. The method was based on the oxidation of promazineby Ce(IV) in sulfuric acidic media resulting in a spectrophotometrically detectablespecies at 512 nm. A33full factorial design and response surface methods wereapplied to optimize experimental conditions potentially controlling the analysis. Theoptimum conditions obtained were1.0×10−4 M sulphuric acid, 0.01 M Ce(IV), and 10 μL/s flow rate. Good analytical parameters were obtained including range of linearity 1–150 μg/mL, linearity with correlation coefficient 0.9997, accuracy with meanrecovery 98.2%, repeatability with RSD 1.4% (n=7consequent injections), intermediate precision with RSD 2.1% (n=5runs over a week), limits of detection0.34 μg/mL, limits of quantification 0.93 μg/mL, and sampling frequency 23 samples/h. The obtained results were realized by the British Pharmacopoeia method and comparable results were obtained. The provided SIA method enjoys the advantages of the technique with respect to rapidity, reagent/sample saving, and safety in solution handling and to the environment.

[学科分类] 分析化学