Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy

[摘要] Background.The purpose of the present study was to assess the feasibility of using miR-126 in the urine as a biomarker for diabetic nephropathy.Methods.miRNAs were extracted from the urine samples of T2DM patients with diabetic nephropathy (DN;n=92), T2DM without DN (n=86), and 85 healthy volunteers using quantitative reverse transcriptase polymerase chain reaction (real-time polymerase chain reaction) analysis. Stability of urinary miR-126 and factors that affected the stability were assessed. A subgroup analysis was also carried out to compare the urinary miR-126 level in T2DM patients well controlled by the treatment versus those who were not well controlled.Results.Urinary miR-126 was stable when the urine samples were kept at room temperature for extended period of time, 4°C, −20°C, and −80°C for up to 12 hours or subjected to 10 freeze-and-thaw cycle. Urinary miR-126 was significantly higher in T2DM patients with DN (5.76±0.33versus3.25±0.45in T2DM patients without DN). Successful treatment significantly reduced urinary miR-126 in T2DM patients with DN to3.89±0.52(P<0.05).Conclusion.miR-126 in the urine is stable and it could be used as a biomarker of DN and to monitor the treatment response.

[学科分类] 内分泌与代谢学