The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations

[摘要] Regular monitoring of mosquito vector populations is an integral component of most vector control programmes. Contemporary data on mosquito species composition, infection status, and resistance to insecticides are a prerequisite for effective intervention. For this purpose we, with funding from the Innovative Vector Control Consortium (IVCC), have developed a suite of high-throughput assays based on a single “closed-tube” platform that collectively comprise the “Vector Population Monitoring Tool” (VPMT). The VPMT can be used to screen mosquito disease vector populations for a number of traits includingAnopheles gambiae s.l.andAnopheles funestusspecies identification, detection of infection withPlasmodiumparasites, and identification of insecticide resistance mechanisms. In this paper we focus on theAnopheles-specific assays that comprise the VPMT and include details of a new assay forresistance todieldrin Rdldetection. The application of these tools, general and specific guidelines on their use based on field testing in Africa, and plans for further development are discussed.

[学科分类] 传染病学