Effective Concentration and Detection ofCryptosporidium,Giardia, and the Microsporidia from Environmental Matrices

[摘要] Cryptosporidiumspp.,Giardiaspp., and members of Microsporidia are enteropathogenic parasites of humans and animals, producing asymptomatic to severe intestinal infections. To circumvent various impediments associated with current detection methods, we tested a method providing multistage purification and separation in a single, confined step.Standard real-time PCR was used as a detection method. Samples spiked withC. parvumandG. intestinaliswere split for comparison to standard Method 1623. Results were equivalent to immunomagnetic procedures forCryptosporidium, andGiardia. Overall percent recovery forCryptosporidiumwith Method 1623 averaged 26.89% (std 21.44%; min = 0%; max = 73%) and was similar but less variable for qPCR method at an estimated average of 27.67 (std 17.65%; min = 5%; max = 63%).ForGiardia, Method 1623 had an overall average recovery of 27.11% (std 17.98%; min = 1%; max = 58%), while multistage purification and qPCR had an estimated lower overall recovery at 18.58% (std 13.95%; min = 0%; max = 35%). Microsporidia were also readily detected with an estimated recovery of 46.81% overall (std 17.66%; min = 18%; max = 70%) forE. intestinalisand 38.90% (std 14.36%; min = 13%; max = 62%) forE. bieneusi.

[学科分类] 医学微生物学