Genotypic Characterization ofYersinia enterocoliticaBiotype 4/O:3 Isolates from Pigs and Slaughterhouses Using SE-AFLP, ERIC-PCR, and PFGE

[摘要] Yersinia enterocoliticais a foodborne pathogen that causes illness in humans and animals. The biotype 4/O:3 has been commonly associated with yersiniosis and is characterized by the presence of chromosomal and extra-chromosomal virulence genes. Molecular typing methods have been successfully used to characterizeY. enterocoliticagenetic heterogeneity and to study the epidemiology of the bacteria from different origins. In this study, 320Y. enterocoliticabiotype 4/O:3 isolates originating in pigs and slaughterhouses were characterized according to the virulence profile, and 61 isolates were typified through SE-AFLP, ERIC-PCR, and PFGE techniques. The majority of the isolates originated from pigs, and the predominant virulence profile wasail+virF+rfbC+ystA+, representing 83.4% of the tested isolates. All of theY. enterocolitica4/O:3 isolates were positive for at leastystAgene. The SE-AFLP and ERIC-PCR patterns were highly homogeneous. The SE-AFLP was more discriminative than the ERIC-PCR and tended to cluster isolates according to the slaughterhouse. Despite the limited genetic diversity ofY. enterocolitica4/O:3, PFGE was shown to be the most discriminative technique considering one band of difference. Fattening pigs proved to be an important reservoir ofY. enterocoliticabiotype 4/O:3 carrying virulence genes.

[学科分类] 医学微生物学