A New Generation Microarray for the Simultaneous Detection and Identification ofYersinia pestisandBacillus anthracisin Food

[摘要] The use of microarrays as a multiple analytic system has generated increased interest and provided a powerful analytical tool for the simultaneous detection of pathogens in a single experiment. A wide array of applications for this technology has been reported. A low density oligonucleotide microarray was generated from the genetic sequences ofY. pestisandB. anthracisand used to fabricate a microarray chip. The new generation chip, consisting of 2,240 spots in 4 quadrants with the capability of stripping/rehybridization, was designated as “Y-PESTIS/B-ANTHRACIS 4x2K Array.” The chip was tested for specificity using DNA from a panel of bacteria that may be potentially present in food. In all, 37 uniqueY. pestis-specific and 83B. anthracis-specific probes were identified. The microarray assay distinguishedY. pestisandB. anthracisfrom the other bacterial species tested and correctly identified theY. pestis-specific oligonucleotide probes using DNA extracted from experimentally inoculated milk samples. Using a whole genome amplification method, the assay was able to detect as low as 1 ng genomic DNA as the start sample. The results suggest that oligonucleotide microarray can specifically detect and identifyY. pestisandB. anthracisand may be a potentially useful diagnostic tool for detecting and confirming the organisms in food during a bioterrorism event.

[学科分类] 医学微生物学