Study on existence of the fisheries resources abundance by using environmental deoxyribonucleic acid (e-DNA) approach at fishing grounds in the Sulawesi Sea, Indonesia

[摘要] Here, we report the results of our preliminary study on deep sea eDNA at fishing ground to approach the fisheries resources abundance at Sulawesi sea by using deep-sea water sampling collected from 10 sites ranging from 110m-200m in depth at front side of the International Coelacanth Research Center and Museum Base at Lolak Waters and Manado Bay North of Sulawesi using Nansen Bottle Sampler (1500 cc). The collected waters were filtered using Power Water Sterivex DNA Isolation Kits and preserved with the DNAiso Reagent then transported to Center for Strategy Research Project, University of the Ryukyus, Okinawa Japan where eDNA analyses were conducted. Our results revealed that the concentrations of eDNA has a good quality were measured with a NanoDrop Lite spectrophotometer, indicating eDNA was successfully extracted. Therefore, by using universal primers for eDNA, MiFish-U-F/R for the 1st-PCR (mt-12S amplification) and 2nd-PCR (tag-indexing) for library preparation to accommodate sequence variations and show that intense signal of MiFish eDNA amplification. Using a high-throughput Illumina MiSeq platform for sequencing analyses, we detected eDNA from 40 fish's species with dominantly by Caranx sexfasciatus, Encrasicholina punctifer.

[发布机构] Faculty of Fisheries and Marine Sciences, Sam Ratulangi University, Indonesia^1;Tropical Biosphere Research Center, University of the Ryukyus, Japan^2;Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Indonesia^3;Faculty of Fisheries and Marine Sciences, Diponegoro University, Indonesia^4;Center of Oceanography, Indonesian Institute of Sciences, Indonesia^5;Aquamarine Fukushima and Marine Sciences Museum, Iwaki, Japan^6;Center for Strategic Research Project, University of the Ryukyus, Japan^7;Faculty of Medicine, University of the Ryukyus, Japan^8

[学科分类] 计算机科学（综合）

[关键词] DNA isolation;Fishing ground;High throughput;Intense signals;Research center;Sequence variations;Sequencing analysis;Strategy research